Microdevices for dielectrophoretic flow-through cell separation

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Microdevices for dielectrophoretic flow-through cell separation - Engineering in Medicine and Biology Magazine, IEEE

M any research groups have developed dielectrophoretic (DEP)-based separation systems for a wide range of particle types, including cells, bacteria, viruses and proteins [1]–[10]. Simple twodimensional (2-D) spatial separation of a binary mixture of particles on a microelectrode array is relatively easy to demonstrate; however, the implementation of a practical flowthrough separation system is ...

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Cell separation by dielectrophoretic field-flow-fractionation.

Dielectrophoretic field-flow-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34+ hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped ...

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Dielectrophoretic separation of bioparticles in microdevices: a review.

In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological en...

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Cellular Separation via Dielectrophoretic Field-Flow-Fractionation

I present a brief review of a cellular separation technique known as dielectrophoretic field-flowfractionation. The technique relies upon suspending different cells at different heights within a laminar flow profile. The differentially suspended cells, thus, travel at different speeds and elute from the flow chamber at different times resulting in successful separation. An alternate fabrication...

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Automated Dielectrophoretic Characterization for Microfluidic Cell Separation Devices

We present a novel analysis technique for automated characterization of particles and cells using dielectrophoresis (DEP). We combine an automated experimental system to measure DEP particle trapping via fluorescence intensity with physically informed computational methods and sophisticated particle models to make robust measurements of DEP force magnitude. The system uses a LabVIEW-controlled ...

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ژورنال

عنوان ژورنال: IEEE Engineering in Medicine and Biology Magazine

سال: 2003

ISSN: 0739-5175

DOI: 10.1109/memb.2003.1266051